Biosynthesis of Mannophospholipids by Mycobacterium phlei

Genetic transfer in Mycobacterium phlei.

Isolation from Mycobacterium Phlei

Mycobactin is the name suggested for a growth factor, present in acid-fast bacteria, which is essential for the growth of Mycobacterium johnei. A preliminary account ofthe work reported here has already been published (Francis, Madinaveitia, Macturk & Snow, 1949). Myco. johnei is an organism first observed by Johne & Frothingham (1895) in cases of chronic enteritis of cattle. It could not be cu...

Citrate formation in Mycobacterium phlei.

Although it is well known that citrate stimulates the growth of acid fast bacteria, the metabolic role of citrate is still obscure in this bacterial group. Washed suspensions of mycobacteria either fail to oxidize citrate or oxidize this substance slowly (Edson, 1951). On the other hand, clarified extracts of mycobacteria oxidize citrate to a-ketoglutarate (Geronimus, Gray, and Birkeland, 1949;...

Conversion of carotenoids to oxycarotenoids by Mycobacterium phlei.

Information concerning the biological interconversions of carotenoids is fragmentary mainly because the methods for their separation and identification are inadequate. Several hypotheses have been presented. Zechmeister and Sandoval (1946) proposed that the newly discovered phytofluene might be a precursor of the carotenoid pigments. Porter and Lincoln (1950) suggested that the more saturated (...

The Mycobacterium phlei Genome: Expectations and Surprises

Mycobacterium phlei, a nontuberculosis mycobacterial species, was first described in 1898-1899. We present the complete genome sequence for theM. phlei CCUG21000(T)type strain and the draft genomes for four additional strains. The genome size for all five is 5.3 Mb with 69.4% Guanine-Cytosine content. This is ≈0.35 Mbp smaller than the previously reported M. phlei RIVM draft genome. The size di...